ORIGINAL ARTICLE
 
KEYWORDS
ABSTRACT
Two new Micarea species, M. minuta and M. pseudotsugae, are described from the Netherlands based on morphological, chemical and molecular data. Micarea minuta belongs to the M. denigrata group and can be distinguished from other similar species of this group by its small apothecia, Sedifolia-grey pigment in the epihymenium, mostly 1-septate and small ascospores, 40–80 μm wide mesopycnidia, and the lack of secondary lichen metabolites detectable by thin-layer chromatography. Micarea pseudotsugae is a member of the M. prasina group and is characterized by an areolate, granular to subsquamulose thallus, the presence of methoxymicareic acid, the lack of Sedifolia-grey pigment, the presence of crystals (visible in polarized light) in the apothecia only, and one type of conidia (mesoconidia). The phylogenetic position of the newly described species was studied based on mtSSU rDNA marker analysis; M. minuta was found to be closely related to M. denigrata, M. nitschkeana and M. subnigrata, while M. pseudotsugae was found to be closely related to M. byssacea and M. laeta. Notes on secondary chemistry, ecology, distribution and phylogeny are given.
 
REFERENCES (37)
1.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. 1990. Basic Local Alignment Search Tool. Journal of Molecular Biology 215: 403–410.
 
2.
Andersen, H. L. 2004. Phylogeny and classification of Micarea. Ph.D. thesis, University of Bergen, Norway.
 
3.
Andersen, H. L. & Ekman, S. 2005. Disintegration of the Micareaceae (lichenized Ascomycota): a molecular phylogeny based on mitochondrial rDNA sequences. Mycological Research 109: 21–30.
 
4.
Aptroot, A. & Cáceres, M. E. D. 2014. New lichen species from termite nests in rainforest in Brazilian Rondonia and adjacent Amazonas. The Lichenologist 46: 365–372.
 
5.
Brand, A. M., van den Boom, P. P. G. & Sérusiaux, E. 2014. Unveiling a surprising diversity in the lichen genus Micarea (Pilocarpaceae) in Réunion (Mascarenes archipelago, Indian Ocean). The Lichenologist 46: 413‒439.
 
6.
Castresana, J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17: 540‒552.
 
7.
Coppins, B. J. 1983. A taxonomic study of the lichen genus Micarea in Europe. Bulletin of the British Museum (Natural History), Botany Series 11: 17‒214.
 
8.
Czarnota, P. 2007. The lichen genus Micarea (Lecanorales, Ascomycota) in Poland. Polish Botanical Studies 23: 1–199.
 
9.
Czarnota, P. & Guzow-Krzemińska, B. 2010. A phylogenetic study of the Micarea prasina group shows that Micarea micrococca includes three distinct lineages. The Lichenologist 42: 7‒21.
 
10.
Dereeper, A., Guignon, V., Blanc, G., Audic, S., Buffet, S., Che­venet, F., Dufayard, J. F., Guindon, S., Lefort, V., M. Lescot, Claverie, J. M. & Gascuel, O. 2008. Phylogeny.fr: robust phylogeetic analysis for the non-specialist. Nucleic Acids Research 36: W465–W469.
 
11.
Guzow-Krzemińska, B. & Węgrzyn, G. 2000. Potential use of restriction analysis of PCR-amplified DNA fragments in construction of molecular data-based identification keys of lichens. Mycotaxon 76: 305–313.
 
12.
Guzow-Krzemińska, B., Czarnota, P., Łubek, A. & Kukwa, M. 2016. Micarea soralifera sp. nov., a new sorediate species in the M. prasina group. The Lichenologist 48: 161–169.
 
13.
Guzow-Krzemińska, B., Sérusiaux, E., van den Boom, P. P. G., Brand, A. M., Launis, A., Łubek, A. & Kukwa, M. 2019a. Understanding the evolution of phenotypical characters in the Micarea prasina group (Pilocarpaceae) and descriptions of six new species within the group. Myco­Keys 57: 1–30.
 
14.
Guzow-Krzemińska, B., Flakus, A., Kosecka, M., Jabłońska, A., Rodriguez-Flakus, P. & Kukwa, M 2019b. New species and new records of lichens from Bolivia. Phytotaxa 397: 257–279.
 
15.
Huelsenbeck, J. P. & Ronquist, F. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754–755.
 
16.
Hyde, K. D., Tennakoon, D. S., Jeewon, R., Bhat, D. J., Maharachchikumbura, S. S. N., Rossi, W., Leonardi, M., Lee, H. B., Mun, H. Y., Houbraken, J., Nguyen, T. T. T., Jeon, S. J., Frisvad, J. C., Wanasinghe, D. N., Lücking, R., Aptroot, A., Caceres, M. E. S., Karunarathna, S. C., Hongsanan, S., Phookamsak, R., Silva, N. I., Thambugala, K. M., Jayawardena, R. S., Senanayake, I. C., Boonmee, S., Chen, J., Luo, Z. L., Phukhamsakda, C., Pereira, O. L., Abreu, V. P., Rosado, A. W. C., Buyck, B., Randrianjohany, E., Hofstetter, V., Gibertoni, T. B., Silva Soares, A. M., Plautz Jr., H. L., Pontes Sotão, H. M., Xavier, W. K. S., Bezerra, J. D. P., Oliveira, T. G. L., Souza-Motta, C. M., Magalhães, O. M. C., Bundhun, D., Harishchandra, D., Manawasinghe, I. S., Dong, W., Zhang, S. N., Bao, D. F., Samarakoon, M. C., Pem, D., Karunarathna, A., Lin, C. G., Yang, J., Perera, R. H., Kumar, V., Huang, S. K., Dayarathne, M. C., Ekanayaka, A. H., Jayasiri, S. C., Xiao, Y. P., Konta, S., Niskanen, T., Liimatainen, K., Dai, Y. C., Ji, X. H., Tian, X. M., Mešić, A., Singh, S. K., Phutthacharoen, K., Cai, L., Sorvongxay, T., Thiyagaraja, V., Norphanphoun, C., Chaiwan, N., Lu, Y. Z., Jiang, H. B., Zhang, J. F., Abeywickrama, P. D., Aluthmuhandiram, J. V. S., Brahmanage, R. S., Zeng, M., Chethana, T., Wei, D. P., Réblová, M., Fournier, J., Nekvindová, J., Barbosa, R. N., Felinto dos Santos, J. E., Oliveira, N. T., Li, G. J., Ertz, D., Shang, Q. J., Phillips, A. J. L., Kuo, C. H., Camporesi, E., Bulgakov, T. S., Lumyong, S., Jones, E. B. G., Chomnunti, P., Gentekaki, E., Bungartz, F., Zeng, X. Y., Fryar, S., Tkalčec, Z., Liang, J. M., Li, G. S., Wen, T. C., Singh, P. N., Gafforov, Y., Promputtha, I., Yasanthika, E., Goonasekara, I. D., Zhao, R. L., Zhao, Q., Kirk, P. M., Liu, J. K., Yan, J. Y., Mortimer, P. E., Xu, J. C. & Doilom, M. W. 2019. Fungal diversity notes 1036–1150: taxonomic and phylogenetic contributions on genera and species of fungal taxa. Fungal Diversity 96: 1–242.
 
17.
Kantvilas, G. 2018. Micarea kartana sp. nov. (lichenized Ascomycetes) from Kangaroo Island, South Australia. Swainsona 31: 55–58.
 
18.
Landan, G. & Graur, D. 2008. Local reliability measures from sets of co-optimal multiple sequence alignments. Pacific Symposium on Biocomputing 13: 15–24.
 
19.
Launis, A. & Myllys, L. 2019. Micarea fennica, a new lignicolous lichen species from Finland. Phytotaxa 409: 179–188.
 
20.
Launis, A., Malícek, J., Sérusiaux, E., Svensson, M., Tsurykau, A. & Myllys, L. 2019a. Sharpening species boundaries in the Micarea prasina group with notes on the type species M. prasina. Mycologia 111: 574–592.
 
21.
Launis, A., Pykälä, J., van den Boom, P. P. G., Sérusiaux, E. & Myllys, L. 2019b. Four new epiphytic species in the Micarea prasina group from Europe. The Lichenologist 51: 7–25.
 
22.
Miadlikowska, J., Kauff, F., Högnabba, F., Oliver, J. C., Molnár, K., Fraker, E., Gaya, E., Hafellner, J., Hofstetter, V., Gueidan, C., Otálora, M. A. G., Hodkinson, B., Kukwa, M., Lücking, R., Björk, C., Sipman, H. J. M., Burgaz, A. R., Thell, A., Passo, A., Myllys, L., Goward, T., Fernández-Brime, S., Hestmark, G., Lendemer, J., Lumbsch, H. T., Schmull, M., Schoch, C. L., Sérusiaux, E., Maddison, D. R., Arnold, A. E., Lutzoni, F. & Stenroos, S. 2014. A multigene phylogenetic synthesis for the class Lecanoromycetes (Ascomycota): 1307 fungi representing 1139 infrageneric taxa, 317 genera and 66 families. Molecular Phylogenetics and Evolution 79: 132–168.
 
23.
Miller, M. A., Pfeiffer, W., Schwartz, T. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, 1–8.
 
24.
Orange, A., James, P. W. & White, F. J. 2001. Microchemical methods for the identification of lichens. British Lichen Society, London.
 
25.
Penn, O., Privman, E., Ashkenazy, H., Landan, G., Graur, D. & Pupko, T. 2010. GUIDANCE: a web server for assessing alignment confidence scores. Nucleic Acids Research 38 (Web Server issue): W23–W28.
 
26.
Rambaut, A. 2012 FigTree v1.4.2. http://tree.bio.ed.ac.uk/softw....
 
27.
Ronquist, F. & Huelsenbeck, J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.
 
28.
Schmull, M., Miadlikovska, J., Pelzer, M., Stocker-Wörgötter, E., Hoffstetter, V., Franker, E., Hodkingson, B., Reeb V., Kukwa, M., Lumbsch, H. T., Kauff, F. & Lutzoni, F. 2011 Phylogenetic affiliations of members of the heterogeneous lichen-forming fungi of the genus Lecidea sensu Zahlbruckner (Lecanoromycetes, Ascomycota). Mycologia 103: 983–1003.
 
29.
Sela, I., Ashkenazy, H., Katoh, K. & Pupko, T. 2015. GUIDANCE2: accurate detection of unreliable alignment regions accounting for the uncertainty of multiple parameters. Nucleic Acids Research 43 (Web Server issue): W7–W14.
 
30.
Sérusiaux, E., Brand, A. M., Motiejūnaitè, J., Orange, A. & Coppins, B. J. 2010. Lecidea doliiformis belongs to Micarea, Catillaria alba to Biatora and Biatora ligni-mollis occurs in western Europe. The Bryologist 113: 333–344.
 
31.
Stamatakis, A. 2014. RAxML Version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313.
 
32.
Svensson, M., Ekman, S., Klepsland, J. T., Nordin, A., Thor, G., von Hirschheydt, G., Jonsson, F., Knutsson, T., Lif, M., Spribille, T. & Westberg, M. 2017. Taxonomic novelties and new records of Fennoscandian crustose lichens. MycoKeys 25: 51‒86.
 
33.
Van den Boom, P. P. G. & Ertz, D. 2014. A new species of Micarea (Pilocarpaceae) from Madeira growing on Usnea. The Lichenologist 46: 295–301.
 
34.
Van den Boom, P. P. G., Brand, A. M., Coppins, B. J. & Sérusiaux, E. 2017a. Two new species in the Micarea prasina group from Western Europe. The Lichenologist 49: 13–25.
 
35.
Van den Boom, P. P. G., Sipman, H. J. M., Divakar, P. K. & Ertz, D. 2017b. New or interesting records of lichens and lichenicolous fungi from Panama, with descriptions of ten new species. Sydowia 69: 47–72.
 
36.
Van den Boom, P. P. G., Brand, A. M., Coppins, B. J. & Sérusiaux, E. 2018. A new Micarea species from western Europe, belonging in the Micarea denigrata group. Herzogia 31: 385–389.
 
37.
Zoller, S., Scheidegger, C. & Sperisen, C. 1999. PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. The Lichenologist 31: 511–516.
 
 
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