ORIGINAL ARTICLE
 
KEYWORDS
ABSTRACT
In a continued effort to dismantle the Sticta fuliginosa morphodeme of western North America, we performed an extensive sampling throughout the mountainous regions of the southwestern states of the USA: Arizona, Colorado, and New Mexico. Recent studies have concluded the genus Sticta has extensive hidden biodiversity and is a large sub-cosmopolitan species-rich genus that encompasses 500+ taxa and counting. The prior treatment, incorporating all laminal isidiate species of Sticta into the Sticta fulignosa s.str. and Sticta sylvatica s.str. morphodemes, was incorrect. Our data does not support those previous treatments nor the broad application of European species concepts of isidiate Sticta in Western North America. Instead, our 4-locus phylogenetic analyses support a narrowing of that concept to a single species of Sticta that is neither S. fuliginosa s.str. nor S. sylvatica s.str. and is a distinct species with a specialized ecological niche. This unique proposed species is named and described here as Sticta puebloensis sp. nov.
REFERENCES (44)
1.
Bandelt, H., Forster, P. & Röhl, A. 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution 16(1): 37–48. https://doi.org/10.1093/oxford....
 
2.
Dal Forno, M., Moncada, B. & Lücking, R. 2018. Sticta aongstroemii, a newly recognized species in the S. damicornis morphodeme (Lobariaceae) potentially endemic to the Atlantic Forest in Brazil. The Lichenologist 50(6): 691–696. https://doi.org/10.1017/S00242....
 
3.
Di Meglio, J. R. & Goward, T. 2023. Resolving the Sticta fuliginosa Morphodeme (Lichenized Ascomycota: Peltigeraceae) in North-western North America. The Bryologist 126(1): 90–110. https://doi.org/10.1639/0007-2....
 
4.
Ekman, S., Tønsberg, T. & Jørgensen, P. M. 2019. The Sticta fuliginosa group in Norway and Sweden. Graphis Scripta 31(4): 23–33. https://hdl.handle.net/1956/22....
 
5.
Galloway, D. J. 1995. Studies on the Lichen Genus Sticta (Schreber) Ach. 3. Notes on Species Described by Bory-De-St-Vincent, Hooker, William, and Delise, Between 1804 and 1825. Nova Hedwigia 61(1–2): 147–188.
 
6.
Galloway, D. J. & Thomas, M. A. 2004. Sticta. In: Nash III, T. H., Ryan, B. D., Diederich, P., Gries, C., Bungartz, F. (eds), Lichen Flora of the Greater Sonoran Desert Region. Vol. II, pp. 513–524. Lichens Unlimited, Arizona State University, Tempe.
 
7.
Guindon, S., Dufayard, J. F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59(3): 307–321. https://doi.org/10.1093/sysbio....
 
8.
Hodkinson, B. P., Lendemer, J. C., McDonald, T. & Harris, R. C. 2014. The Status of Sticta sylvatica, an ‘Exceedingly Rare’ Lichen Species, in Eastern North America. Evansia 31(1): 17–24. https://doi.org/10.1639/079.03....
 
9.
Katoh, K., Misawa, K., Kuma, K. I. & Miyata, T. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30(14): 3059–3066. https://doi.org/10.1093/nar/gk....
 
10.
Katoh, K. & Standley, D. M. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780. https://doi.org/10.1093/molbev....
 
11.
Lendemer, J. C. & Goffinet, B. 2016. Sticta deyana: a new endemic photomorphic lichen from the imperiled Mid-Atlantic Coastal Plain of eastern North America. Systematic Botany 40(4): 933–941. https://doi.org/10.1600/036364....
 
12.
Lindgren, H., Moncada, B., Lücking, R., Magain, N., Simon, A., Goffinet, B., Sérusiaux, E., Nelsen, M. P., Mercado-Díaz, J. A., Widhelm, T. J. & Lumbsch, H. T. 2020. Cophylogenetic patterns in algal symbionts correlate with repeated symbiont switches during di-versification and geographic expansion of lichen-forming fungi in the genus Sticta (Ascomycota, Peltigeraceae). Molecular Phy-logenetics and Evolution 150: 106860. https://doi.org/10.1016/j.ympe....
 
13.
Magain, N. & Sérusiaux, E. 2015. Dismantling the treasured flagship lichen Sticta fuliginosa (Peltigerales) into four species in Western Europe. Mycological Progress 14: 1–33. https://doi.org/10.1007/s11557....
 
14.
Martini, M. A., Kaplan, M. R., Strelin, J. A., Astini, R. A., Schaefer, J. M., Caffee, M. W. & Schwartz, R. 2017. Late Pleistocene glacial fluctuations in Cordillera oriental, subtropical Andes. Quaternary Science Reviews 171: 245–259. https://doi.org/10.1016/j.quas....
 
15.
McDonald, T., Miadlikowska, J. & Lutzoni, F. 2003. The lichen genus Sticta in the Great Smoky Mountains: a phylogenetic study of morphological, chemical, and molecular data. The Bryologist 106(1): 61–79. https://doi.org/10.1639/0007-2....
 
16.
Mercado‐Díaz, J. A., Lücking, R., Moncada, B., Widhelm, T. J. & Lumbsch, H. T. 2020. Elucidating species richness in lichen fungi: The genus Sticta (Ascomycota: Peltigeraceae) in Puerto Rico. Taxon 69(5): 851–891. https://doi.org/10.1002/tax.12....
 
17.
Moncada, B. 2012. El Género Sticta (Schreb.) Ach. en Colombia: Taxonomía, Ecogeografía e Importancia. Doctoral Dissertation, Universidad Nacional de Colombia, Bogotá D.C., Colombia.
 
18.
Moncada, B. & Lücking, R. 2012. Ten new species of Sticta and counting: Colombia as a hot spot for unrecognized diversification in a conspicuous macrolichen genus. Phytotaxa 74: 1–29.
 
19.
Moncada, B., Coca, L. F. & Lücking, R. 2013a. Neotropical members of Sticta (lichenized Ascomycota: Lobariaceae) forming photo-symbiodemes, with the description of seven new species. The Bryologist 116(2): 169–200. https://doi.org/10.1639/0007-2....
 
20.
Moncada, B., Lücking, R. & Betancourt-Macuase, L. 2013b. Phylogeny of the Lobariaceae (lichenized Ascomycota: Peltigerales), with a reappraisal of the genus Lobariella. The Lichenologist 45(2): 203–263. https://doi.org/10.1017/S00242....
 
21.
Moncada, B., Lücking, R. & Coca, L. F. 2013c. Six new apotheciate species of Sticta (lichenized Ascomycota: Lobariaceae) from the Colombian Andes. The Lichenologist 45(5): 635–656. https://doi.org/10.1017/S00242....
 
22.
Moncada, B., Lücking, R. & Suárez, A. 2014. Molecular phylogeny of the genus Sticta (lichenized Ascomycota: Lobariaceae) in Colombia. Fungal Diversity 64: 205–231. https://doi.org/10.1007/s13225....
 
23.
Moncada, B., Suárez, A. & Lücking, R. 2015. Nueve especies nuevas del género Sticta (Ascomycota liquenizados: Lobariaceae) del morfotipo fuliginosa sensu lato de Colombia. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 39: 50–66. https://doi.org/10.18257/racce....
 
24.
Moncada, B., Mercado-Díaz, J. A. & Lücking, R. 2018. The identity of Sticta damicornis (Ascomycota: Lobariaceae): a presumably widespread taxon is a Caribbean endemic. The Lichenologist 50(5): 591–597. https://doi.org/10.1017/S00242....
 
25.
Moncada, B., Lücking, R. K. & Lumbsch, H. T. 2020. Rewriting the evolutionary history of the lichen genus Sticta (Ascomycota: Peltigeraceae subfam. Lobarioideae) in the Hawaiian Islands. Plant and Fungal Systematics 65(1): 95–119. https://doi.org/10.35535/pfsys....
 
26.
Moncada, B., Smith, C. W. & Lücking, R. 2021a. A taxonomic reassessment of the genus Sticta (lichenized Ascomycota: Peltigeraceae) in the Hawaiian archipelago. The Lichenologist 53(1): 117–133. https://doi.org10.1017/S002428....
 
27.
Moncada, B., Mercado-Díaz, J. A., Smith, C. W., Bungartz, F., Sérusiaux, E., Lumbsch, H. T. & Lücking, R. 2021b. Two new common, previously unrecognized species in the Sticta weigelii morphodeme (Ascomycota: Peltigeraceae). Willdenowia 51(1): 35–45. https://doi.org/10.3372/wi.51.....
 
28.
Moncada, B., Rincón-Murillo, D. & Lücking, R. 2023. Three new lobarioid lichens (lichenized Ascomycota: Peltigeraceae) from Colombia, honoring Enrique Forero. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 47(184): 619–640. https://doi.org/10.18257/racce....
 
29.
Ossowska, E. A. 2021. First records of Sticta weigelii s.str. from Bolivia confirmed by molecular data. Folia Cryptogamica Estonica 58: 65–72. https://doi.org/10.12697/fce.2....
 
30.
Ossowska, E. A., Moncada, B., Kukwa, M., Flakus, A., Rodriguez-Flakus, P., Olszewska, S. & Lücking, R. 2022a. New species of Sticta (lichenised Ascomycota, lobarioid Peltigeraceae) from Bolivia suggest a high level of endemism in the Central Andes. ­MycoKeys 92: 131–160. https://doi.org/10.3897/mycoke....
 
31.
Ossowska, E., Kosecka, M., Jaskólska, J. & Kukwa, M. 2022b. Two taxa of the genus Sticta (Peltigerales, Ascomycota), S. andina and S. scabrosa subsp. scabrosa, new to Bolivia confirmed by molecular data. Plant and Fungal Systematics 67(2): 45–54. https://doi.org/10.35535/pfsys....
 
32.
Ossowska, E. A., Moncada, B., Lücking, R., Flakus, A., Rodriguez-Flakus, P., Olszewska, S. & Kukwa, M. 2024. Additional new species and new records of the genus Sticta (lichenized Ascomycota, lobarioid Peltigeraceae) from Bolivia. MycoKeys 105: 21–47. https://doi.org/10.3897/mycoke....
 
33.
Rehner, S. A. & Samuels, G. J. 1994. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Fungal Biology 98: 625–634. https://doi.org/10.1016/S0953-....
 
34.
Ronquist, F. & Huelsenbeck, J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19(12): 1572–1574. https://doi.org/10.1093/bioinf....
 
35.
Simon, A., Goward, T., Di Meglio, J., Dillman, K., Spribille, T. & Goffinet, B. 2018. Sticta torii sp. nov., a remarkable lichen of high conservation priority from northwestern North America. Graphis Scripta 30(6): 105–114. https://hdl.handle.net/2268/22....
 
36.
Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post–analysis of large phylogenies. Bioinformatics 30(9): 1312–1313. https://doi.org/10.1093/bioinf....
 
37.
Suarez, A. & Lücking, R. 2013. Sticta viviana (lichenized Ascomycota: Peltigerales: Lobariaceae), a new species from Colombian paramos. The Lichenologist 45(2): 153–157. https://doi.org/10.1017/S00242....
 
38.
Torres, J. M., Barbosa, T. D., Kitaura, M. J., Spielmann, A. A. & Lorenz, A. P. 2021. Two new species of Sticta (Peltigeraceae subfam. Lobarioideae) from the Brazilian Cerrado (Brazilian savanna). The Bryologist 124(4): 506–521. https://doi.org/10.1639/0007-2....
 
39.
Van der Hammen, T. 1989. History of the montane forests of the northern Andes. In Woody plants – evolution and distribution since the Tertiary: Proceedings of a symposium organized by Deutsche Akademie der Naturforscher Leopoldina in Halle/Saale, German Democratic Republic, October 9–11 1986: 109–114. Springer Vienna.
 
40.
Vilgalys, R. & Hester, M. 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 178(8): 4238–4246. https://doi.org/10.1128/jb.172....
 
41.
White, T. J., T. Burns, S. Lee & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: a guide to methods and applications 18(1): 315–322.
 
42.
Widhelm, T. J., Bertoletti, F. R., Asztalos, M. J., Mercado-Diaz, J. A., Huang, J. P., Moncada, B., Lücking, R., Magain, N., Serusiaux, E., Goffinet, B. & Crouch, N. 2018. Oligocene origin and drivers of diversification in the genus Sticta (Lobariaceae, Ascomyco-ta). Molecular Phylogenetics and Evolution 126: 58–73. https://doi.org/10.1016/j.ympe....
 
43.
Yánez-Ayabaca, A., Benítez, Á., Molina, R. B., Naranjo, D., Etayo, J., Prieto, M., Cevallos, G., Caicedo, E., Scharnagl, K., McNerlin, B. & Swanson, S. 2023. Towards a dynamic checklist of lichen-forming, lichenicolous and allied fungi of Ecuador – using the Consortium of Lichen Herbaria to manage fungal biodiversity in a megadiverse country. The Lichenologist 55(5): 203–222. https://doi.org/10.1017/S00242....
 
44.
Zoller, S., Scheidegger, C. & Sperisen, C. 1999. PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. The Lichenologist 31(5): 511–516. https://doi.org/10.1006/lich.1....
 
eISSN:2657-5000
ISSN:2544-7459
Journals System - logo
Scroll to top