ORIGINAL ARTICLE
Three distinctive Preussia (Sporormiaceae) from photosynthetic stems of Ephedra trifurca (Ephedraceae, Gnetophyta) in southeastern Arizona, USA
1
Department of Ecology and Evolutionary Biology, The University of
Arizona, Tucson, AZ 85721, USA
2
School of Plant Sciences, The University of Arizona, Tucson, AZ
85721, USA
3
Facultad de Ciencias, Universidad Nacional Autónoma de México,
Ciudad Universitaria, CDMX, México
Online publication date: 2022-12-28
Publication date: 2022-12-28
Plant and Fungal Systematics 2022; 67(2): 63-74
KEYWORDS
ABSTRACT
A survey of the diversity and community composition of culturable endophytic
fungi associated with photosynthetic stems of the gnetophyte Ephedra trifurca (Ephedraceae)
in southeastern Arizona, USA, yielded numerous isolates that are consistent morphologically
with members of the genus Preussia (Sporormiaceae, Pleosporales, Dothideomycetes, Ascomycota).
Preussia (including species until recently classified within Sporormiella) include
dung-, soil-, and plant-inhabiting strains with considerable diversity worldwide. We used
morphological evidence and phylogenetic analyses (nuclear ribosomal internal transcribed
spacers and 5.8s gene, the adjacent D1-D2 region of the nuclear ribosomal large subunit,
and for a subset of strains, the translation elongation factor 1-alpha, EF1-a) to identify
distinctive members of the Preussia intermedia clade occurring as endophytes in E. trifurca.
These include P. arizonica sp. nov., which also occurs as an endophyte in other plants of
the region, and P. elegans sp. nov., which has been found only in E. trifurca to date. We
also propose Preussia mariae sp. nov., allied phylogenetically with Preussia lignicola but
distinguishable on the basis of morphology and EF1-a data. Our analyses illustrate the
potential for several currently recognized species of Preussia to represent species complexes
that should be resolved by analyses of additional loci and by further sampling of
endophytes, which may provide an ecological connection among strains occurring within
living plant tissues and as coprophilous or soil-inhabiting fungi. More broadly, our work
expands the known geographic scope, host use, and diversity of Preussia, especially in arid
lands. In conjunction with previous work, our study also provides the basis for hypotheses
regarding secondary metabolites of the newly described species.
FUNDING
Financial support for this work was provided by the College of
Agriculture and Life Sciences at the University of Arizona.
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