Differential responses to salt concentrations of lichen
photobiont strains isolated from lichens occurring in
different littoral zones
| 1 | Departamento de Ciencias de la Vida, Universidad de Alcalá, Campus
Externo, 28805, Alcalá de Henares, Spain |
| 2 | Museo Nacional de Ciencias Naturales (CSIC), Calle Serrano 115-
dpdo, 28006, Madrid, Spain |
| 3 | Real Jardín Botánico (CSIC), Plaza Murillo 2, 28014 Madrid, Spain |
Publication date: 2019-12-16
Plant and Fungal Systematics 2019; 64(2): 149–162
KEYWORDS
ABSTRACT
An interesting biota of lichen-forming fungi occurs along rocky seashores of cold
and warm-temperate regions in both hemispheres. Most of the species belong to the family
Verrucariaceae and form symbioses with an extraordinarily diverse group of photobionts.
We isolated the photobionts of three species: Hydropunctaria maura and H. amphibia from
the supralittoral zone, and Wahlenbergiella striatula from the upper intertidal zone. We
characterized the isolated strains structurally by means of transmission electron microscopy,
and molecularly using the nrSSU and nrITS and chloroplast RPL10A regions. Additionally,
we studied the response of the strains to different salt concentrations, analyzed the
concentration of osmoregulatory solutes, and measured photosynthesis performance by
chlorophyll fluorescence and CO2 assimilation techniques. All strains belong to the recently
described species Halofilum ramosum, although we found differences in the ITS and RPL10A
regions among the strains shared by H. maura and H. amphibia and the strain isolated
from W. striatula. Differences were also found in the main osmoregulatory response of the
strains growing under high salt concentrations: W. striatula accumulated glycerol, while
H. maura and H. amphibia synthetized sucrose. Analyses of photosynthesis performance
also indicated differences in physiological behavior between supralittoral-dwelling and
intertidal-dwelling species, W. striatula showing lower photosynthetic activity under high
irradiance. Our results highlight the role of photobionts in determining lichen zonation on
rocky seashores.
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