ORIGINAL ARTICLE
Chemical diversity of five coastal Roccella species from mainland France, the Scattered Islands, and São Tomé and Príncipe
| 1 | Univ Rennes, CNRS, ISCR (Institut des Sciences Chimiques de
Rennes) – UMR 6226, F-35000 Rennes, France |
| 2 | UMS 2006 PatriNat (OFB – CNRS – MNHN), Muséum national
d’Histoire naturelle, Maison Buffon, CP41, 36 rue Geoffroy Saint-
Hilaire 75005 Paris |
Online publication date: 2020-12-29
Publication date: 2020-12-29
Plant and Fungal Systematics 2020; 65(2): 247–260
KEYWORDS
Roccella applanataRoccella belangerianaRoccella fuciformisRoccellamontagneiRoccella phycopsischemical profile
ABSTRACT
Roccella species constitute interesting models to address questions regarding
lichen metabolite diversity across taxonomic, ecological and geographic gradients. Indeed,
owing to their wide distribution, their taxonomic diversity and the narrow ecological niche
they occupy, Roccella species are good candidates to study the drivers of lichen chemistry.
This study focuses on the chemical profiling of five species: R. applanata, R. belangeriana,
R. fuciformis, R. montagnei and R. phycopsis. These five species were sampled in a rather
narrow longitudinal range (1°51′W to 47°17′E) covering the Eastern Atlantic and Western
Indian Ocean areas along an extended latitudinal range (48°49′N to 22°23′S). High Pressure
Liquid Chromatography (HPLC) analysis followed by mass spectrometry of 31 Roccella
thalli revealed a number of interesting patterns through a multivariate (PCA) analysis,
including the first detailed chemical profiles for two species from the Scattered Islands:
R. applanata and R. belangeriana. Metabolite segregation amongst all studied Roccella
species, including R. montagnei and R. belangeriana, gave some insight into the taxonomy
of the latter two species, which we interpret as separate species. An additional analysis
focusing on R. montagnei samples revealed chemical differences along both a latitudinal
and ecological gradient (from Europa Island to São Tomé and Príncipe). Three mass spectra
databases were built to dereplicate the ions, which gave an overview of the factors that
could drive quantitative and qualitative metabolite composition in lichens. Additionally,
several new Roccella species records are reported for the Scattered Islands, as well as São
Tomé and Príncipe.
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